Strain Data Sheet

RBRC10067

Information1

Image
BRC No.RBRC10067
TypeTransgene and Spontaneous MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-c/c Tg(CAG-iRFP)#846Utr
Former Common nameiRFP Tg #846
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yoshihiro Miwa, University of Tsukuba in 2012. A DNA construct was injected into fertilized eggs derived from B6D2F1. Mice were backcrossed to B6(Cg)Tyr<c2-J> for 17 generations. C57BL/6J congenic line.
Strain descriptionTransgenic mice expressing iRFP under the control of the CAG promoter. This line (B6.Cg-c/c Tg(CAG-iRFP)#846Utr) exhibits stronger iRFP expression in heart than RBRC10068 (B6.Cg-c/c Tg(CAG-iRFP)#867Utr).
Colony maintenance
ReferencesExp. Anim., 63, 311-319 (2014). 25077761

Health Report

Examination Date / Room / Rack2020/11/16Room:3-DRack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tyrtyrosinase7Tyr<c-2J>albino 2 Jackson

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
iRFPphytochrome-based near-infrared fluorescent protein (iRFP)UNiRFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Rhodopseudomonas palustris iRFP DNA, SV40 Poly(A) site
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 63, 311-319 (2014).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR Dr. Yoshihiro Miwa (ymiwa@md.tsukuba.ac.jp) of University of Tsukuba using the Approval Form.
DepositorYoshihiro Miwa (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Feb 2019
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data