Strain Data Sheet

RBRC10064

Information1

Image
BRC No.RBRC10064
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Foxp3<tm2.1(EGFP/Cre)Shori>
Former Common nameB6.Foxp3eGFPCre
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shohei Hori, RIKEN Center for Integrative Medical Sciences. M1 ES cells (C57BL/6Jx129/Sv F1) were used. The neo gene was removed by crossing to CAG-Cre mice, and then the mice were backcrossed to C57BL/6J over 12 generations. C57BL/6 congenic line.
Strain descriptionKnockin mice expressing eGFP-Cre under the control of the Foxp3 (forkhead box P3) gene locus. A DNA fragment containing IRES and eGFP-Cre was knocked in at 3’ UTR (between stop codon and endogenous polyA) of the Foxp3 gene.
Colony maintenance
ReferencesImmunity. 2012 Feb 24;36(2):262-75. doi: 10.1016/j.immuni.2011.12.012. Epub 2012 Feb 9. 22326580

Health Report

Examination Date / Room / Rack2022/12/12Room:3-6Rack:D
2022/09/12Room:3-6Rack:D
2022/06/13Room:3-6Rack:D
2022/04/18Room:3-6Rack:D
2022/03/14Room:3-6Rack:D
2021/12/14Room:3-6Rack:D
2021/09/13Room:3-6Rack:D
2021/06/14Room:3-6Rack:D
2021/03/29Room:3-6Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Foxp3forkhead box P3XFoxp3<tm2.1(EGFP/Cre)Shori>targeted mutation 2.1, Shohei Hori

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)X

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)X

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseXcre

Information2

Donor DNAECMV (Encephalomyocarditis virus) IRES, Aequorea victoria GFP (eGFP) cDNA, Enterobacteria phage P1 Cre, Saccharomyces cerevisiae Frt site, mouse Foxp3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity. 2012 Feb 24;36(2):262-75. doi: 10.1016/j.immuni.2011.12.012. Epub 2012 Feb 9.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorShohei Hori (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data