Strain Data Sheet

RBRC10063

Information1

Image
BRC No.RBRC10063
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Foxp3<tm1(CD2/CD52)Shori>
Former Common nameB6.Foxp3hCD2
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shohei Hori, RIKEN Center for Integrative Medical Sciences. C57BL/6J-derived ES cells were used. The neo gene was removed by crossing to CAG-Cre mice. C57BL/6J genetic background.
Strain descriptionFoxp3 (forkhead box P3) reporter mice. A DNA fragment containing IRES and cDNA for human CD2-human CD52 fusion protein was knocked in at 3’ UTR (between stop codon and endogenous polyA) of the Foxp3 gene.
Colony maintenance
ReferencesImmunity. 2012 Feb 24;36(2):262-75. doi: 10.1016/j.immuni.2011.12.012. Epub 2012 Feb 9. 22326580

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Foxp3forkhead box P3XFoxp3<tm1(CD2/CD52)Shori>targeted mutation 1, Shohei Hori

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CD2CD2 (human)XCD2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CD52CD52 antigenXCD52

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)X

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Information2

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), human CD2 cDNA, human CD52 cDNA, Enterobacteria phage P1 loxP site, mouse Foxp3 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity. 2012 Feb 24;36(2):262-75. doi: 10.1016/j.immuni.2011.12.012. Epub 2012 Feb 9.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorShohei Hori (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data