Strain Data Sheet

RBRC10062

Information1

Image
BRC No.RBRC10062
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-F9<em1Tsuka>
Former Common nameF9 knockout mice (CRISPR/Cas9)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tsukasa Ohmori, Jichi Medical University. C57BL/6J background.
Strain descriptionF9 (coagulation factor IX) mutant mice. F9 activity is less than 1% in homozygous mice. Deleted sequence: ATATACTAAGGTTTCCCGGTACGTCAACTGGATTAA.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2020/11/16Room:3-DRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F9coagulation factor IXXF9<em1Tsuka>endonuclease-mediated mutation 1, Tsukasa Ohmori

Information2

Donor DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorTsukasa Ohmori (Jichi Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Jun 2019
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data