Strain Data Sheet

RBRC10061

Strain Information

Image
BRC No.RBRC10061
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-F8<tm1.2(EGFP)Tsuka>
Former Common nameF8 EGFP knockin mice
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Tsukasa Ohmori, Jichi Medical University. Bruce 4 ES cell line was used. C57BL/6 congenic line.
Strain descriptionEGFP knock-in mice at the F8 (coagulation factor VIII) gene locus. EGFP was placed at downstream of Exon 16. F8 activity is less than 1% in homozygous mice.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)XEGFP
F8
MGI:88383		coagulation factor VIIIXF8<tm1.2(EGFP)Tsuka> targeted mutation 1.2, Tsukasa Ohmori
  • hemophilia A(DisGeNET, MedGEN)
    • P2A porcine teschovirus-1 2A peptideXP2A
    loxP phage P1 loxPXloxP
    SV40 polyAX

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, SA, mouse F8 cDNA, P2A (porcine teschovirus-1 2A peptide), jellyfish EGFP cDNA, Simian Virus (SV40) PolyA, mouse F8 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Mouse Models for Human Disease
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorTsukasa Ohmori (Jichi Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data