RBRC10061

Information1

Image
BRC No.RBRC10061
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-F8<tm1.2(EGFP)Tsuka>
Former Common nameF8 EGFP knockin mice
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack2020/06/01Room:3-DRack:K
2020/02/17Room:3-DRack:K
2019/12/16Room:3-DRack:K
2019/10/21Room:3-DRack:K
2019/08/26Room:3-DRack:K
2019/06/17Room:3-DRack:K
2019/04/15Room:3-DRack:K
2019/02/18Room:3-DRack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
F8coagulation factor VIIIXF8<tm1.2(EGFP)Tsuka>targeted mutation 1.2, Tsukasa Ohmori

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)XEGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
P2Aporcine teschovirus-1 2A peptideXP2A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyAX

Information2

Donor DNAPhage P1 loxP site, SA, mouse F8 cDNA, Picornavirus P2A, jellyfish EGFP cDNA, Simian Virus (SV40) PolyA, mouse F8 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorTsukasa Ohmori (Jichi Medical University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data