Strain Data Sheet

RBRC10060

Information1

Image
BRC No.RBRC10060
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-F8<tm1.1(EGFP)Tsuka>
Former Common nameF8 EGFP knockin flox/flox mice
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Tsukasa Ohmori, Jichi Medical University. Bruce 4 ES cell line was used. C57BL/6 congenic line.
Strain descriptionF8 (coagulation factor VIII) floxed mice with an inducible EGFP expression. Exon 16 of F8 is replaced with the following DNA fragment; loxP-F8 cDNA (exon 16–28)-SV40 pA-loxP-splice acceptor-P2A-EGFP-SV40 pA. In the presence of Cre, cDNA (exon 16–28) can be deleted and EGFP starts to express. Even in the absence of Cre, there is some EGFP expression due to splicing. RBRC10061 is a strain in which Cre deleted DNA fragment between loxP sites.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F8coagulation factor VIIIXF8<tm1.1(EGFP)Tsuka>targeted mutation 1.1, Tsukasa Ohmori

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)XEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptideXP2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAX

Information2

Donor DNAPhage P1 loxP sites, SA, mouse F8 cDNA, Simian Virus (SV40) PolyA, Saccharomyces cerevisiae Frt site, P2A (porcine teschovirus-1 2A peptide), jellyfish EGFP cDNA, mouse F8 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorTsukasa Ohmori (Jichi Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data