Strain Data Sheet

RBRC10054

Information1

Image
BRC No.RBRC10054
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Aqp4<tm1Tsna>
Former Common nameAQP4 Neo, Aqp4 flox
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrl
Appearance
Strain developmentDeveloped by Mika Tsujita, Brain Research Institute, Niigata University. RENKA ES cells derived from C57BL/6 were used. C57BL/6 genetic background.
Strain descriptionAqp4 mutant mice in which loxP-FRT-pgk-neo-FRT DNA fragment was inserted between exon 1 and exon 2, and another loxP was inserted between exon 3 and exon 4.
Colony maintenance
ReferencesNeurosci. Res., 64, 208-212 (2009). 19428702

Health Report

Examination Date / Room / Rack2021/11/15Room:3-CRack:A
2021/08/16Room:3-CRack:A
2021/05/17Room:3-CRack:A
2021/02/15Room:3-CRack:A
2020/11/16Room:3-CRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Aqp4aquaporin 418Aqp4<tm1Tsna>targeted mutation 1, Tsutomu Nakada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)18mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal18

Information2

Donor DNAphage P1 loxP sites, Saccharomyces cerevisiae FRT sites, mouse Pgk1 promoter, E. coli aminoglycoside 3'-phosphotransferase, Simian virus 40 SV40 pA, mouse Aqp4 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 64, 208-212 (2009).
DepositorMika Terumitsu-Tsujita (Niigata University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Sep 2017
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data