Strain Data Sheet

RBRC10053

Information1

Image
BRC No.RBRC10053
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6(Cg)-Aqp4<tm1.1Tsna>
Former Common nameAQP4 KO, Aqp4-
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Mika Tsujita, Brain Research Institute, Niigata University. RENKA ES cells derived from C57BL/6 were used to generate a strain (RBRC10054) harboring a floxed Aqp4 allele with the neo resistance gene. By crossing with a TLCN Cre mouse strain, exon 2, 3 and neo were deleted. C57BL/6 genetic background.
Strain descriptionAqp4 (aquaporin 4) knockout mice.
Colony maintenance
ReferencesNeurosci. Res., 64, 208-212 (2009). 19428702

Health Report

Examination Date / Room / Rack2022/07/25Room:3-CRack:G
2022/04/25Room:3-CRack:G
2022/02/14Room:3-CRack:G
2021/11/15Room:3-CRack:G
2021/08/16Room:3-CRack:G
2021/05/17Room:3-CRack:G
2021/02/15Room:3-CRack:G
2020/11/16Room:3-CRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Aqp4aquaporin 418Aqp4<tm1.1Tsna>targeted mutation 1.1, Tsutomu Nakada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Information2

Donor DNAPhage P1 loxP site, mouse Aqp4 genomic DNA
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 64, 208-212 (2009).
DepositorMika Terumitsu-Tsujita (Niigata University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Mouse of the Month Sep 2017
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data