RBRC10052

Information1

Image
BRC No.RBRC10052
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-S1pr2<tm1.1Toka>
Former Common nameS1pr2-venus
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development
Strain descriptionThe first 498 bp of the S1pr2 protein coding region was replaced with the Venus gene. A loxP-flanked PGK-Neo cassette was inserted before exon 2, and was deleted later. S1pr2 gene expression can be analyzed by flow cytometry. Fluorescence of Venus in some cells such as germinal center B cells is detectable by microscopy but is not very bright unless cells/tissues are fixed and stained with anti-GFP and a fluorescent dye-labeled secondary antibody.Homozygous mice are S1pr2-deficient.
Colony maintenance
ReferencesJ. Exp. Med., 211(7), 1297-1305 (2014). 24913235

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
S1pr2sphingosine-1-phosphate receptor 29S1pr2<tm1.1Toka>targeted mutation 1.1, Takaharu Okada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
venusGreen Fluorescent Protein, F46L (Jellyfish)9venus

Information2

Donor DNABacterial virus (Enterobacteria phage P1) LoxP site, Aequorea victoria GFP variant (Venus) cDNA, mouse S1pr2 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 211(7), 1297-1305 (2014).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: sakurai-h@brain.riken.jp).
DepositorTakaharu Okada (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Approval form Form V (English)

BRC mice in Publications

No Data