Strain Data Sheet
RBRC10052
Information1 | |
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| Image | |
| BRC No. | RBRC10052 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-S1pr2<tm1.1Toka> |
| Former Common name | S1pr2-venus |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | Developed by Takaharu Okada, RIKEN Research Center for Allergy and Immunology around 2009-2011. B6N/B6J F1-hybrid ES cells were used and mice were backcrossed to C57BL/6J for 4 generations. C57BL/6 genetic background. |
| Strain description | The first 498 bp of the S1pr2 protein coding region was replaced with the Venus gene. A loxP-flanked PGK-Neo cassette was inserted before exon 2, and was deleted later. S1pr2 gene expression can be analyzed by flow cytometry. Fluorescence of Venus in some cells such as germinal center B cells is detectable by microscopy but is not very bright unless cells/tissues are fixed and stained with anti-GFP and a fluorescent dye-labeled secondary antibody. Homozygous mice are S1pr2-deficient. About 40 % of homozygous mutants die around 3-5 weeks old. |
| Colony maintenance | Heterozygote x Wild-type [or Crossing to C57BL/6JJcl] |
| References | J. Exp. Med., 211(7), 1297-1305 (2014). 24913235 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)9Venus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP |
Information2 | |
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| Donor DNA | Enterobacteria phage P1 LoxP site, Aequorea victoria GFP variant (Venus) cDNA, mouse S1pr2 genomic DNA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 211(7), 1297-1305 (2014).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp). |
| Depositor | Takaharu Okada (RIKEN) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |