RBRC10017

Information1

Image
BRC No.RBRC10017
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Lcmt1<tm1d(EUCOMM)Hmgu>/C09
Former Common name
H-2 Haplotype
ES Cell lineJM8A3.N1 [C57BL/6N-A<tm1Brd>]
Background strainC57BL/6NJcl
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / Rack2018/04/16Room:3-DRack:A
2018/02/19Room:3-DRack:A
2017/12/18Room:3-DRack:A
2017/10/16Room:3-DRack:A
2017/08/21Room:3-DRack:A
2017/06/19Room:3-DRack:A
2017/04/17Room:3-DRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Lcmt1leucine carboxyl methyltransferase 17Lcmt1<tm1e(EUCOMM)Hmgu>targeted mutation 1e, Helmholtz Zentrum Muenchen GmbH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)7

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)7

Information2

Donor DNAyeast FRT (flipase recombination target) sites, En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, SV40 polyadenylation site, phage P1 loxP sites, hBactP (human beta actin promoter), E. coli neo, mouse Lcmt1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data