Strain Data Sheet

RBRC10016

Information1

Image
BRC No.RBRC10016
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Rhoj<tm1Maim>/76Maim
Former Common name
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by a group at Nagoya City University and Osaka University at 2006. D3 ES cell line was used. Mice were backcrossed to C57BL/6J for 18 generations. C57BL/6 congenic strain.
Strain descriptionRhoj(ras homolog family member J, TCL/TC10bL)knockout mice.
Colony maintenance
ReferencesJ. Biol. Chem., 278, 15279-15284 (2003). 12578843
Biol. Pharm. Bull., 33, 404-409 (2010). 20190400

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rhojras homolog family member J12Rhoj<tm1 Maim>targeted mutation 1, Masayoshi Imagawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)12mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomysin resistant gene, mouse PGK polyA, mouse Rhoj genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorMakoto Nishizuka (Nagoya City University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data