Strain Data Sheet

RBRC10015

Information1

Image
BRC No.RBRC10015
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Bcl6<tm1.1Toka>
Former Common nameYFP-Bc16
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Takaharu Okada, RIKEN Research Center for Allergy and Immunology around 2007-2009. Bruce 4 ES cells were used and mice were backcrossed to C57BL/6J for 5 generations.
Strain descriptionThe monomeric yellow fluorescent protein (YFP) gene was inserted in-frame right after the initiation codon of the Bcl6 gene such that the product of this allele would be the YFP-Bcl6 fusion protein. Bcl6 protein expression can be analyzed by flow cytometry. Fluorescence of YFP-Bcl6 in most most cell types is too dim to be analyzed by microscopy unless cells/tissues are stained with anti-GFP and a fluorescent dye-labeled secondary antibody. YFP-Bcl6 is functionally compromised, possibly because fusion with YFP near the BTB domain of Bcl6 might impair recruitment of the corepressor molecules or disrupt the formation of Bcl6 homodimers or heterodimers with other BTB domain-containing transcription factors. Therefore, homozygous animals are hypomorphic. Heterozygous animals are largely normal.
Colony maintenance
ReferencesImmunity, 34(6), 961-972 (2011). 21636294

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bcl6B cell leukemia/lymphoma 616Bcl6<tm1.1Toka>targeted mutation 1.1, Takaharu Okada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)16EYFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Information2

Donor DNAphage P1 LoxP site, Aequorea victoria GFP variant(EYFP)cDNA, mouse Bcl6 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 34(6), 961-972 (2011).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTakaharu Okada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Jan 2019
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data