Strain Data Sheet

RBRC10005

Information1

Image
BRC No.RBRC10005
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;Cg-Col1a1<tm1(tetO-TACSTD2<*>)Hirt>
Former Common nameTet-O-TACSTD2 intracellular domain
H-2 Haplotype
ES Cell lineKH2[F1:C57BL/6 x 129Sv]
Background strain
Appearance
Strain developmentDeveloped by Hiroyuki Tomita, Gifu University in 2010. KH2 ES cell (F1:C57BL/6 x 129/SV) was used. Mice were backcrossed to C57BL/6J for 3 or 4 generations.
Strain descriptionA mouse strain in which cDNA coding intracellular domain of Tacstd2 (tumor-associated calcium signal transducer 2) with FLAG tag is driven by the TetO promoter. DNA construct was knocked in at the Col1a1 (collagen, type I, alpha 1) locus.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Col1a1collagen, type I, alpha 111Col1a1<tm1(tetO-Tacstd2<*>)Hirt>targeted mutation 1, Hiroyuki Tomita

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)11FLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hyghygromycin phasphotransferase (E. coli)11Hyg

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TACSTD2tumor-associated calcium signal transducer 2 (human)11TACSTD2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA11

Information2

Donor DNAyeast FRT (flipase recombination target) sites, Adenovirus Major late transcript splice acceptor sequence, E. coli tetO (tetracycline operator), human TACSTD2 cDNA, Synthetic FLAG tag, SV40 polyA, vector, Escherichia coli ampicillin resistant gene, mouse PGK promoter, E. coli hygromycin resistance gene, mouse Col1a1 genomic DNA
Research applicationFLP/frt system
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroyuki Tomita (Gifu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data