Strain Data Sheet

RBRC10003

Information1

Image
BRC No.RBRC10003
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameICR;Cg-Hsf1<tm1Anak>
Former Common nameHSF1KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Sachiye Inoue and Akira Nakai, Yamaguchi University in 2003. TT2 ES cells(C57BL/6 x CBA F1 origin)were used. Mice were backcrossed to ICR.
Strain descriptionHsf1 (heat shock transcription factor 1) knockout mice. Homozygous females are infertile.
Colony maintenance
ReferencesMol. Cell Biol., 23, 5882-5895 (2003). 12897157

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hsf1heat shock transcription factor 115Hsf1<tm1Anak>targeted mutation 1, Akira Nakai

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)15mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase I (PGK) promoter, E. coli neomycin resistance gene, mouse phosphoglycerate kinase I (PGK) polyA signal, mouse Hsf1 genomic DNA
Research applicationCancer Research
Neurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 23, 5882-5895 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAkira Nakai (Yamaguchi University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Jul 2019
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data