Strain Data Sheet

RBRC10002

update : 2024-09-20

Strain Information
Image
BRC No.	RBRC10002
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6.Cg-Maml1<tm1Mkit>
Former Common name	MamL1, Mastermind-like-1, Mastermind-1, Mam-1, mKIAA0200
H-2 Haplotype
ES Cell line	E14.1 [129P2/OlaHsd]
Background strain	C57BL/6JJcl
Appearance
Strain development	Developed by Motoo Kitagawa and his colleagues, Chiba University and The University of Tokyo. E14-1 (129P2/OlaHsd) ES cells were used. Mice were backcrossed to C57BL/6 for more than 20 generations.
Strain description	Maml1 (mastermind like 1) knockout mice. Exon 1 was replaced with the neomycin resistance gene. Homozygous mutants are postnatally lethal.
Colony maintenance
References	Notch signalling in the nucleus: roles of Mastermind-like (MAML) transcriptional coactivators. Kitagawa M J. Biochem., 159, 287-294 (2016). 26711237 MAML1 enhances the transcriptional activity of Runx2 and plays a role in bone development. Watanabe T, Oyama T, Asada M, Harada D, Ito Y, Inagawa M, Suzuki Y, Sugano S, Katsube K, Karsenty G, Komori T, Kitagawa M, Asahara H PLoS Genet. 2013;9(1):e1003132. doi: 10.1371/journal.pgen.1003132. 23326237 Olfactory Sensory Neurons Control Dendritic Complexity of Mitral Cells via Notch Signaling. Muroyama Y, Baba A, Kitagawa M, Saito T PLoS Genet. 2016 Dec 27;12(12):e1006514. doi: 10.1371/journal.pgen.1006514. 28027303 Mastermind-like 1 (MamL1) and mastermind-like 3 (MamL3) are essential for Notch signaling in vivo. Oyama T, Harigaya K, Sasaki N, Okamura Y, Kokubo H, Saga Y, Hozumi K, Suganami A, Tamura Y, Nagase T, Koga H, Nishimura M, Sakamoto R, Sato M, Yoshida N, Kitagawa M Development, 138, 5235-5246 (2011). 22069191 The repression of Notch signaling occurs via the destabilization of mastermind-like 1 by Mesp2 and is essential for somitogenesis. Sasaki N, Kiso M, Kitagawa M, Saga Y Development, 138, 55-64 (2011). 21098559 Mastermind-1 is required for Notch signal-dependent steps in lymphocyte development in vivo. Oyama T, Harigaya K, Muradil A, Hozumi K, Habu S, Oguro H, Iwama A, Matsuno K, Sakamoto R, Sato M, Yoshida N, Kitagawa M Proc. Natl. Acad. Sci. USA, 104, 9764-9769 (2007). 17535917

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Maml1	mastermind like transcriptional coactivator 1	11	Maml1	targeted mutation 1, Motoo Kitagawa
neo	neomycin resistance gene (E. coli)	11				mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype
Annotation by Mammalian phenotyhpe ontology	decreased body weight(MP:0001262) decreased marginal zone B cell number(MP:0008182) decreased thymocyte number(MP:0000715) dystrophic muscle(MP:0000752) increased double-negative T cell number(MP:0005090) more 2 phenotypes postnatal growth retardation(MP:0001732) postnatal lethality, incomplete penetrance(MP:0011086)
Detailed phenotype data

Ordering Information
Donor DNA	mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistance gene, mouse Pgk1 polyA signal, mouse Maml1 genomic DNA
Research application
Specific Term and Conditions	Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 104, 9764-9769 (2007). Development, 138, 5235-5246 (2011).
Depositor	Motoo Kitagawa (Chiba University)
Strain Status	Frozen embryos Frozen sperm
Strain Availability	Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month)
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Genotyping protocol -PCR-

BRC mice in Publications

No Data