Strain Data Sheet

RBRC10002

Information1

Image
BRC No.RBRC10002
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Maml1<tm1Mkit>
Former Common nameMamL1, Mastermind-like-1, Mastermind-1, Mam-1, mKIAA0200
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Motoo Kitagawa and his colleagues, Chiba University and The University of Tokyo. E14-1 (129P2/OlaHsd) ES cells were used. Mice were backcrossed to C57BL/6 for more than 20 generations.
Strain descriptionMaml1 (mastermind like 1) knockout mice. Exon 1 was replaced with the neomycin resistance gene. Homozygous mutants are postnatally lethal.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 104, 9764-9769 (2007). 17535917
PLoS Genet. 2013;9(1):e1003132. doi: 10.1371/journal.pgen.1003132. 23326237
Development, 138, 5235-5246 (2011). 22069191
Development, 138, 55-64 (2011). 21098559
J. Biochem., 159, 287-294 (2016). 26711237
PLoS Genet. 2016 Dec 27;12(12):e1006514. doi: 10.1371/journal.pgen.1006514. 28027303

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Maml1mastermind like 1 (Drosophila)11Maml1<tm1Mkit>targeted mutation 1, Motoo Kitagawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistance gene, mouse Pgk1 polyA signal, mouse Maml1 genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 104, 9764-9769 (2007). Development, 138, 5235-5246 (2011).
DepositorMotoo Kitagawa (Chiba University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data