Strain Data Sheet

RBRC09994

Strain Information

Image
BRC No.RBRC09994
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Eno2-GFP/Atg5)1Nmz
Former Common nameNse-Atg5
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Noboru Mizushima, Graduate School and Faculty of Medicine, The University of Tokyo. C57BL/6J genetic background.
Strain descriptionTransgenic mice expressing GFP-Atg5 fusion protein under the control of the rat Eno2/Nse(neuron specific enolase)promoter.
Colony maintenance
References
Systemic Analysis of Atg5-Null Mice Rescued from Neonatal Lethality by Transgenic ATG5 Expression in Neurons.
Yoshii S R, Kuma A, Akashi T, Hara T, Yamamoto A, Kurikawa Y, Itakura E, Tsukamoto S, Shitara H, Eishi Y, Mizushima N
Dev. Cell, 39, 116-130 (2016). 27693508

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signalUN
  • spinocerebellar ataxia, autosomal recessive 25(MedGEN)
    • Atg5autophagy-related 5 (yeast)UNAtg5
  • spinocerebellar ataxia, autosomal recessive 25(MedGEN)
    • EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPrat Eno2 promoter
  • spinocerebellar ataxia, autosomal recessive 25(MedGEN)
    • neoneomycin resistance gene (E. coli)UNSV40 promoter
  • spinocerebellar ataxia, autosomal recessive 25(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal Leydig cell differentiation(MP:0013602)

  • abnormal Paneth cell morphology(MP:0004843)

  • abnormal autophagy(MP:0008260)

  • abnormal circulating amino acid level(MP:0005311)

  • abnormal circulating androgen level(MP:0009819)
    • more 47 phenotypes
  • abnormal endometrial gland development(MP:0013400)

  • abnormal eosinophil morphology(MP:0005061)

  • abnormal large intestine crypts of Lieberkuhn morphology(MP:0004842)

  • abnormal liver morphology(MP:0000598)

  • abnormal mesangial cell morphology(MP:0005327)

  • abnormal pancreas morphology(MP:0001944)

  • abnormal parturition(MP:0002907)

  • abnormal skeletal muscle fiber morphology(MP:0003084)

  • abnormal spermatid morphology(MP:0006380)

  • anemia(MP:0001577)

  • blunted small intestinal villi(MP:0014083)

  • decreased body size(MP:0001265)

  • decreased body weight(MP:0001262)

  • decreased brain weight(MP:0002175)

  • decreased circulating iron level(MP:0004151)

  • decreased circulating leptin level(MP:0005668)

  • decreased circulating testosterone level(MP:0002780)

  • decreased circulating unsaturated transferrin level(MP:0011897)

  • decreased erythrocyte cell number(MP:0002875)

  • decreased liver iron level(MP:0008743)

  • decreased ovary weight(MP:0004856)

  • decreased pituitary gland weight(MP:0008938)

  • decreased seminal vesicle weight(MP:0004910)

  • decreased skeletal muscle fiber size(MP:0009400)

  • decreased skeletal muscle weight(MP:0010239)

  • decreased small intestinal villus height(MP:0014082)

  • decreased spleen iron level(MP:0008808)

  • decreased submandibular gland size(MP:0009520)

  • decreased testis weight(MP:0004852)

  • decreased thymus weight(MP:0004956)

  • decreased uterus weight(MP:0004905)

  • decreased white adipose tissue mass(MP:0014146)

  • enlarged liver(MP:0000599)

  • enlarged lymph nodes(MP:0000702)

  • enlarged spleen(MP:0000691)

  • expanded mesangial matrix(MP:0011353)

  • glomerulonephritis(MP:0002743)

  • heterochrony(MP:0014021)

  • increased inflammatory response(MP:0001846)

  • increased neutrophil cell number(MP:0000219)

  • increased plasma cell number(MP:0008097)

  • increased spleen germinal center number(MP:0008481)

  • interstitial pneumonia(MP:0001862)

  • liver degeneration(MP:0003103)

  • oligozoospermia(MP:0002687)

  • pancreas degeneration(MP:0013584)

  • premature aging(MP:0003786)
    • Detailed phenotype data

    Ordering Information

    Donor DNARat Neuron specific enolase promoter, jellyfish Green fluorescent protein (GFP) cDNA, mouse Atg5 cDNA, SV40 polyA additional signal, SV40 early promoter, E. coli neomycin resistance gene
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell 39: 116-130 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 1. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. 2. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 3. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorNoboru Mizushima (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data