Strain Data Sheet

RBRC09994

Information1

Image
BRC No.RBRC09994
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Eno2-GFP/Atg5)1Nmz
Former Common nameNse-Atg5
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Noboru Mizushima, Graduate School and Faculty of Medicine, The University of Tokyo. C57BL/6J genetic background.
Strain descriptionTransgenic mice expressing GFP-Atg5 fusion protein under the control of the rat Eno2/Nse(neuron specific enolase)promoter.
Colony maintenance
ReferencesDev. Cell, 39, 116-130 (2016). 27693508

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Atg5autophagy-related 5 (yeast)UNAtg5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPrat Eno2 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)UNSV40 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Information2

Donor DNARat Neuron specific enolase promoter, jellyfish Green fluorescent protein (GFP) cDNA, mouse Atg5 cDNA, SV40 polyA additional signal, SV40 early promoter, E. coli neomycin resistance gene
Research applicationFluorescent Proteins/lacZ System
Specific Term and Conditions1. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. 2. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 3. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested: Dev. Cell 39: 116-130 (2016). 5. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.　6. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorNoboru Mizushima (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

No Data