Strain Information | |
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Image | |
BRC No. | RBRC09954 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6D2-Frem3<em1Osb> |
Former Common name | Frem3<-5/-5> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Daiji Kiyozumi and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2015. Fertilized eggs derived from BDF1×BDF1 were used. Mixed genetic background. |
Strain description | Frem3 mutant mice generated by the CRISPR/Cas9 technique. 5 bp deletion at exon 1.Frem3<em1Osb>: 5 bp deletion at exon 1.ACTCCAGAATTCCATCGGACTATGGCTGGAGATTCTCTGAGCCTTCCTGGGATGTCCCTGCAGCTGCTAGTGACTCTCACCTGCCTGCTTCTGACTTGTGCACTGAGGGAAAGGGTACCCTTGAGTGAAAACGTGTCCCACTGTGAGCTTTACCTGCCTCCCTGGGGAGCGCTTAATGGTGGTAGTCACCAGAACCCTGGCATTCTGATCGCCAACTCCGGGTTCCAAGTGCCACAGGGCCGCTCGGTTTGGCTTAACCCACTGCGGGATCTAGTGATTCGAGTGCAGCGCGGGGATCAG |
Colony maintenance | |
References | Exp. Anim., 69(1):104-109 (2020). 31554749 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frem3Fras1 related extracellular matrix protein 38Frem3<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University |
Ordering Information | |
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Donor DNA | human hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of Frem3 gene , human hU6 promoter, CMV enhancer (CBh) |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 69(1):104-109 (2020).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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No Data |