Strain Data Sheet

RBRC09938

Information1

Image
BRC No.RBRC09938
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-1110017D15Rik<em1Osb>
Former Common name1110017D15Rik<-4/-4>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Asami Oji and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. Transgene was injected to fertilized eggs derived from BDF1×BDF1. Mixed genetic background.
Strain description1110017D15Rik mutant mice generated by the CRISPR/Cas9 technique. 4 bp deletion at exon 1.1110017D15Rik<em1Osb>: 4 bp deletion at exon 1.GGAAGATACTGAGAGGTCCCCCAAAGGAAGGGGTCACAGCATCTAGGGGCACCTTCCAGGCCGGAGGTGTGGGCTATTTAGGGGAAGGGGTAGTCACAGATGTCCAGCAGGAGTCCTTGAGAGGCAAAGCCCACTACAGCATCCCTGAGACCCTACCATGTTCCTCTTCTCCCGGAAGACCAAGACCCCCATCAGCACCTACAGTGACTCCTACAGGGCTCCTACCTCCATCAAGGAGGTCTATAAGGACCCACCACTATGGGCCTGGGAAGCCAACAAGTTTGTGACCCCAgtaagtagaggctgcaaatgggatggaaggagtatggccgtgtggctgggcagctggcatttggacatctctgtgtttgtagttatttcctgtttcaggttttgttttgttttaatttcatttatgtatttatttacttatttatcgagacaaggcctcactgtgtagcctagcttgacctaaaacttgtgacaatcctacatcagcctcccaagtactggctggaattacaggtaggaaccatgccc
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). 27357688

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
1110017D15RikRIKEN cDNA 1110017D15 gene41110017D15Rik<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Information2

Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of 1110017D15Rik gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data