Strain Data Sheet



BRC No.RBRC09937
SpeciesMus musculus
Strain nameB6D2-Ddx3y<em1Osb> Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb
Former Common nameDdx3y<x/+225>
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Ayako Isotani and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2013. BDF1 background.
Strain descriptionDbx3y mutant mice (225 bp insertion) generated by the CRISPR/Cas9 technique. This strain also has a transgene, Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb (RBRC03743).Ddx3y<em1Osb>: 225 bp insertion catgccctcatctcaatatcccataaggtacaacactaaaccagaatattaacaaagtacaagtcacgttttacttactgaaattttaaattgctaattattaaaagctgttgaaattttgtttgggtatccagtgtctatcactgtactgggatcagttattttagaagtctgtggcaatgaagagactttttgggttttgttctttttttccttgaaagGGGTCTGTGATAAGGACAGTTCAGGATGGAGCTGTAGTAAAGATAAAGATGCCTACAGCAGTTTTGGATCTCGTGATTCCAGAGGGAAGCCCAATTATTTCAGTGATCGTGG(ATCCACATTGGACGGTTGGAGATTGACTTTTGCTTTTGATTGTGGCTGTGCCCTGATATTTTCCCTCTCGAAGGAAGAAACTGTTTTAGTGGAGCCCACAGTTAAGAGACTTTTAATTGTAAAAAGACTTTGGATTTTAAAAGAGATGGCTATTTTAAAGAAATTGAAATTTTAAGAATATGTAAAGACTGTGGGACTTTTAAAGTTATTTAAATCTAATCCACA)AAGTGGATCCAGGGGAAGgtatattcttggttgataatgtacaaagtaatggttaagtatcttagtagttaagaatatgtaagaatcttaacttagcaaagtcagggttctcaaaactgatagaatgaatctgtctacctacttacctaagaatttactaattagagtggtgtacatgttgttgtccaactagtccaacaatggctatcc
Colony maintenance
ReferencesJ. Reprod. Dev., 65(2):121-128 (2019). 30613052

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DsRed2Red Fluorescent Protein (Discosoma sp.)UNDsRed2CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPmouse proacrosin promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Su9mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)]UNSu9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ddx3yDEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linkedYDdx3y<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University8030469F12Rik, D1Pas1-rs1, Dby


Donor DNAchicken beta-actin promoter, rabbit beta-globin polyadenylation signal, CMV CMV IE enhancer, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter, mouse Cdc20b genomic DNA, SV40 polyadenylation site, mouse PGK promoter, E.coli. Neomysin phosphotransferase gene (neoR), Yeast FRT site, Phage P1 loxP site
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Reprod Dev. 2019 Apr 12;65(2):121-128RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
  6. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

No Data