Strain Data Sheet

RBRC09931

Information1

Image
BRC No.RBRC09931
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Prm2<em1(S56A)Kyot>
Former Common namePrm2 S56A
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Katsuhiko Itoh, Graduate School of Medicine and Faculty of Medicine, Kyoto University in 2015. C57BL/6 genetic background.
Strain descriptionA point mutation S56A was intrduced into the Prm2 (protamine 2) gene by the CRISPR/Cas9 technique. Homozygous mice are fertile.
Colony maintenance
ReferencesSci. Signal., 12(574) (2019). 30914484

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prm2protamine 216Prm2<em1(S56A)Kyot>endonuclease-mediated mutation 1, Katuhiki Itoh

Information2

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin]
Research application
Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKatsuhiko Itoh (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data