Strain Data Sheet

RBRC09930

Strain Information

Image
BRC No.RBRC09930
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CAG-GFP/LC3/RFP/LC3<*>deltaG)2Nmz
Former Common nameGFP-LC3-RFP-LC3 delta G#2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Noboru Mizushima, Graduate School and Faculty of Medicine, The University of Tokyo. C57BL/6J genetic background.
Strain descriptionTransgenic mice expressing GFP-LC3 and RFP-LC3ΔG under the control of the CAG promoter. High expression was observed only in the skeletal muscle.
Colony maintenance
References
An Autophagic Flux Probe that Releases an Internal Control.
Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, Kodama A, Ishihara T, Mizushima T, Mizushima N
Mol. Cell, 64, 835-849 (2016). 27818143

Health Report

Examination Date / Room / Rack2024/11/25Room:4-CRack:K
2024/08/26Room:4-CRack:K
2024/05/27Room:4-CRack:K
2024/02/26Room:4-CRack:K
2023/11/27Room:4-CRack:K
2023/08/28Room:4-CRack:K
2023/05/29Room:4-CRack:K
2023/02/27Room:4-CRack:K

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
Map1lc3b microtubule-associated protein 1 light chain 3 betaUNMap1lc3b
RFP Red Fluorescent Protein (Discosoma sp.)UNRFP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
  • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Rat LC3 cDNA, Rat LC3ΔG cDNA, jellyfish Green fluorescent protein(GFP) cDNA, Discosoma sp. Red fluorescent protein (RFP) cDNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell, 64, 835-849 (2016). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorNoboru Mizushima (The University of Tokyo)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. UCSD MTA
    6. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data