Information1 | |
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Image | |
BRC No. | RBRC09930 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | C57BL/6J-Tg(CAG-GFP/LC3/RFP/LC3<*>deltaG)2Nmz |
Former Common name | GFP-LC3-RFP-LC3 delta G#2 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Noboru Mizushima, Graduate School and Faculty of Medicine, The University of Tokyo. C57BL/6J genetic background. |
Strain description | Transgenic mice expressing GFP-LC3 and RFP-LC3ΔG under the control of the CAG promoter. High expression was observed only in the skeletal muscle. |
Colony maintenance | |
References | Mol. Cell, 64, 835-849 (2016). 27818143 |
Health Report | |
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Examination Date / Room / Rack | 2023/08/28Room:4-CRack:K 2023/05/29Room:4-CRack:K 2023/02/27Room:4-CRack:K |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Map1lc3bmicrotubule-associated protein 1 light chain 3 betaUNMap1lc3b Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter RFPRed Fluorescent Protein (Discosoma sp.)UNRFP |
Information2 | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Rat LC3 cDNA, Rat LC3ΔG cDNA, jellyfish Green fluorescent protein(GFP) cDNA, Discosoma sp. Red fluorescent protein (RFP) cDNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | 1. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. 2. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 3. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested: Mol. Cell 64:835-849 (2016). 5. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 6. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Noboru Mizushima (The University of Tokyo) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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No Data |