Strain Information | |
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Image | |
BRC No. | RBRC09930 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | C57BL/6J-Tg(CAG-GFP/LC3/RFP/LC3<*>deltaG)2Nmz |
Former Common name | GFP-LC3-RFP-LC3 delta G#2 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Noboru Mizushima, Graduate School and Faculty of Medicine, The University of Tokyo. C57BL/6J genetic background. |
Strain description | Transgenic mice expressing GFP-LC3 and RFP-LC3ΔG under the control of the CAG promoter. High expression was observed only in the skeletal muscle. |
Colony maintenance | |
References | An Autophagic Flux Probe that Releases an Internal Control. Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, Kodama A, Ishihara T, Mizushima T, Mizushima N Mol. Cell, 64, 835-849 (2016). 27818143 |
Health Report | |
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Examination Date / Room / Rack | 2024/11/25Room:4-CRack:K 2024/08/26Room:4-CRack:K 2024/05/27Room:4-CRack:K 2024/02/26Room:4-CRack:K 2023/11/27Room:4-CRack:K 2023/08/28Room:4-CRack:K 2023/05/29Room:4-CRack:K 2023/02/27Room:4-CRack:K |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | UN | EGFP | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
Map1lc3b | microtubule-associated protein 1 light chain 3 beta | UN | Map1lc3b | ||||
RFP | Red Fluorescent Protein (Discosoma sp.) | UN | RFP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Rat LC3 cDNA, Rat LC3ΔG cDNA, jellyfish Green fluorescent protein(GFP) cDNA, Discosoma sp. Red fluorescent protein (RFP) cDNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell, 64, 835-849 (2016). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Noboru Mizushima (The University of Tokyo) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |