Strain Data Sheet

RBRC09930

Information1

Image
BRC No.RBRC09930
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CAG-GFP/LC3/RFP/LC3<*>deltaG)2Nmz
Former Common nameGFP-LC3-RFP-LC3 delta G#2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Noboru Mizushima, Graduate School and Faculty of Medicine, The University of Tokyo. C57BL/6J genetic background.
Strain descriptionTransgenic mice expressing GFP-LC3 and RFP-LC3ΔG under the control of the CAG promoter. High expression was observed only in the skeletal muscle.
Colony maintenance
ReferencesMol. Cell, 64, 835-849 (2016). 27818143

Health Report

Examination Date / Room / Rack2023/02/27Room:4-CRack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Map1lc3bmicrotubule-associated protein 1 light chain 3 betaUNMap1lc3b

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
RFPRed Fluorescent Protein (Discosoma sp.)UNRFP

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Rat LC3 cDNA, Rat LC3ΔG cDNA, jellyfish Green fluorescent protein(GFP) cDNA, Discosoma sp. Red fluorescent protein (RFP) cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and Conditions1. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. 2. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 3. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 4. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested: Mol. Cell 64:835-849 (2016). 5. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.　6. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorNoboru Mizushima (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. UCSD MTA
  6. Acceptance of responsibility for living modified organism (Japanese / English)
  7. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data