Strain Data Sheet

RBRC09929

Information1

Image
BRC No.RBRC09929
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Xcr1<tm4(cre)Ksho>
Former Common nameXCR1-cre, XCR1-cre B6
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2008.
Strain descriptionA chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-cre mice were generated by knocking the gene encoding cre recombinase into the murine XCR1 gene locus. In XCR1-cre mice, cre recombinase is expressed secifically in XCR1-expressing cells.
Colony maintenance
ReferencesSci. Rep., 6, 23505 (2016). 27005831

Health Report

Examination Date / Room / Rack2022/12/12Room:3-5Rack:C
2022/09/12Room:3-5Rack:C
2022/06/13Room:3-5Rack:C
2022/03/14Room:3-5Rack:C
2021/12/14Room:3-5Rack:C
2021/09/13Room:3-5Rack:C
2021/07/12Room:3-5Rack:C
2021/06/14Room:3-5Rack:C
2021/03/29Room:3-5Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Xcr1chemokine (C motif) receptor 19Xcr1<tm4(cre)Ksho>targeted mutation 4, Tsuneyasu Kaisho

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase9cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNABacteriophage P1 Cre, Bos taurus GH polyA, Saccharomyces cerevisiae FRT sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), Polyomavirus enhancer, Escherichia coli Neomycin resistant gene, HSV TK polyA signal, mouse Xcr1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep. 6:23505 (2016).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorTsuneyasu Kaisho (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data