Strain Data Sheet
RBRC09928
Information1 | |
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| Image | |
| BRC No. | RBRC09928 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Xcr1<tm3(KikGR)Ksho> |
| Former Common name | XCR1-KikGR, B6.Cg-Xcr1<tm3Ksho> |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2008. |
| Strain description | A chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-KikGR mice were generated by knocking the gene encoding a photoconvertible green-to-red fluorescent protein KikGR into the murine XCR1 gene locus. In XCR1-KikGR mice, XCR1-expressing cells can be detected by KikGR expression. |
| Colony maintenance | |
| References | Proc. Natl. Acad. Sci. USA, 113, 1044-1049 (2016). 26755602 |
Health Report | |
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| Examination Date / Room / Rack | 2023/01/10Room:3-BRack:L |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site9Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site9Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter kikGRGreen-Red Fluorescent Protein (Favia favus)9kikGR Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Information2 | |
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| Donor DNA | Favia speciosa KikGR cDNA, Bos taurus GH polyA, Bacteriophage P1 loxP sites, Saccharomyces cerevisiae FRT sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), Polyomavirus enhancer, Escherichia coli Neomycin resistant gene, HSV TK polyA, mouse Xcr1 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System Immunology and Inflammation Research |
| Specific Term and Conditions | 1. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113, 1044-1049 (2016). 3. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and a right holder for phKikGR1-S1, Medical & Biological Laboratories (MBL) Co., Ltd., and must obtain a prior written consent from the DEPOSITOR and MBL. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the kikGR using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp). |
| Depositor | Tsuneyasu Kaisho (RIKEN) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering: |
BRC mice in Publications |
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