Strain Data Sheet

RBRC09928

Strain Information

Image
BRC No.RBRC09928
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Xcr1<tm3(KikGR)Ksho>
Former Common nameXCR1-KikGR, B6.Cg-Xcr1<tm3Ksho>
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2008.
Strain descriptionA chemokine receptor, XCR1, is a receptor for XCL1 and expressed dominantly in one dendritic cell subset. XCR1-KikGR mice were generated by knocking the gene encoding a photoconvertible green-to-red fluorescent protein KikGR into the murine XCR1 gene locus. In XCR1-KikGR mice, XCR1-expressing cells can be detected by KikGR expression.
Colony maintenance
References
Imaging of the cross-presenting dendritic cell subsets in the skin-draining lymph node.
Kitano M, Yamazaki C, Takumi A, Ikeno T, Hemmi H, Takahashi N, Shimizu K, Fraser S E, Hoshino K, Kaisho T, Okada T
Proc. Natl. Acad. Sci. USA, 113, 1044-1049 (2016). 26755602

Health Report

Examination Date / Room / Rack2024/04/08Room:3-BRack:L
2024/01/09Room:3-BRack:L
2023/10/10Room:3-BRack:L
2023/07/10Room:3-BRack:L
2023/04/10Room:3-BRack:L
2023/03/24Room:3-BRack:L
2023/01/10Room:3-BRack:L

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site9Frt
Frtyeast FRT (flippase recombination target) site9Frt
Xcr1chemokine (C motif) receptor 19Xcr1targeted mutation 3, Tsuneyasu Kaisho
kikGRGreen-Red Fluorescent Protein (Favia favus)9kikGR
loxPphage P1 loxP9loxP
loxPphage P1 loxP9loxP
neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
  • Detailed phenotype data

    Ordering Information

    Donor DNAFavia speciosa KikGR cDNA, Bos taurus GH polyA, Bacteriophage P1 loxP sites, Saccharomyces cerevisiae FRT sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), Polyomavirus enhancer, Escherichia coli Neomycin resistant gene, HSV TK polyA, mouse Xcr1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Immunology and Inflammation Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 113, 1044-1049 (2016). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing KikGR, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. PLoS One 3 (12): e3944 (2008).
    DepositorTsuneyasu Kaisho (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data