Strain Data Sheet

RBRC09920

Information1

Image
BRC No.RBRC09920
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Pdx1<em1(cre/ERT2)Rbrc>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentRIKEN BRC developed this strain by the CRISPR/Cas9 technique using fertilized eggs derived from C57BL/6NJcl. This was funded through the FY2016 AMED-NBRP fundamental technology upgrading program.
Strain descriptionCre/ERT2 knockin mice at the Pdx1 gene locus. A 2A-Cre/ERT2 DNA fragment was placed in frame at the C-terminus of the Pdx1 protein coding sequence.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2023/01/23Room:3-DRack:G
2022/10/25Room:3-DRack:G
2022/07/25Room:3-DRack:G
2022/04/25Room:3-DRack:G
2022/02/14Room:3-DRack:G
2021/11/15Room:3-DRack:G
2021/08/16Room:3-DRack:G
2021/05/17Room:3-DRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pdx1pancreatic and duodenal homeobox 15Pdx1<em1(cre/ERT2)Rbrc>endonuclease mediated mutation 1, RIKEN BRC

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)5ERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA5HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide5P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase5cre

Information2

Donor DNAP2A (porcine teschovirus-1 2A peptide), Bacteriophage P1 Cre, Human ERT2 cDNA, Rabbit Beta globin polyA, mouse Pdx1 genomic DNA
Research applicationCre/loxP system
Specific Term and Conditions1, In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to RIKEN BRC is requested.
2, For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
3, RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorRIKEN BRC (RIKEN BRC)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data