Strain Data Sheet

RBRC09908

Information1

Image
BRC No.RBRC09908
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Braf<tm1Tumg>
Former Common nameBraf Q241R Neo/+ (BALB/c background)
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainBALB/cAnNCrlCrlj
Appearance
Strain developmenteveloped by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2014. BRUCE-4 ES cells were used. B6.Cg-Braf<tm1Tumg>(RBRC09730) was backcrossed to BALB/c for 10 generations.
Strain descriptionKnock-in mutants in which Braf-mutated protein (BrafQ241R) is expressed in a Cre-dependent manner. BALB/c genetic background. Q241R mutation in mouse Braf corresponds to Q257R in human BRAF, the most frequent mutation in the cardio-facio-cutaneous (CFC) syndrome. Strains maintained in B6 and 129 genetic background are available as RBRC09730 and RBRC09907, respectively. Mice constitutively expressing BrafQ241R in the ICR genetic background are available as RBRC09731.
Colony maintenance
ReferencesHum. Mol. Genet., 23, 6553-6566 (2014). 25035421

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BrafBraf transforming gene6Braf<tm1(Q241R)Tumg>targeted mutation 1, Yoko Aoki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal6

Information2

Donor DNABacteriophage P1 loxP sites, STOP transcriptional sequence (Adenovirus splice acceptor, SV40 polyA), Saccharomyces cerevisiae FRT sites, Mouse Phosphoglycerate kinase promotor (PGK promotor), E. coli Neomycin resistant gene, Polyomavirus SV40 polyA signal, mouse Braf genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum. Mol. Genet., 23, 6553-6566 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYoko Aoki (Tohoku University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data