Strain Data Sheet

RBRC09907

Information1

Image
BRC No.RBRC09907
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129.Cg-Braf<tm1Tumg>
Former Common nameBraf Q241R Neo/+ (129 background)
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2014. BRUCE-4 ES cells were used. B6.Cg-Braf<tm1Tumg>(RBRC09730) was backcrossed to 129X1/SvJJmsSlc for 8 generations.
Strain descriptionKnock-in mutants in which Braf-mutated protein (BrafQ241R) is expressed in a Cre-dependent manner. 129 genetic background. Q241R mutation in mouse Braf corresponds to Q257R in human BRAF, the most frequent mutation in the cardio-facio-cutaneous (CFC) syndrome. Strains maintained in B6 and BALB/c genetic background are available as RBRC09730 and RBRC09908, respectively. Mice constitutively expressing BrafQ241R in the ICR genetic background are available as RBRC09731.
Colony maintenance
ReferencesHum. Mol. Genet., 23, 6553-6566 (2014). 25035421

Health Report

Examination Date / Room / Rack2022/07/11Room:3-BRack:B
2022/04/11Room:3-BRack:B
2022/01/24Room:3-BRack:B
2021/10/26Room:3-BRack:B
2021/07/26Room:3-BRack:B
2021/04/26Room:3-BRack:B
2021/02/01Room:3-BRack:B
2020/11/09Room:3-BRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
BrafBraf transforming gene6Braf<tm1(Q241R)Tumg>targeted mutation 1, Yoko Aoki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tyrtyrosinase7Tyr<c>albino

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tyrtyrosinase7Tyr<c-ch>chinchilla

Information2

Donor DNABacteriophage P1 loxP sites, STOP transcriptional sequence (Adenovirus splice acceptor, SV40 polyA), Saccharomyces cerevisiae FRT sites, Mouse Phosphoglycerate kinase promotor (PGK promotor), E. coli Neomycin resistant gene, Polyomavirus SV40 polyA signal, mouse Braf genomic DNA
Research applicationCancer Research
Cre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum. Mol. Genet., 23, 6553-6566 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYoko Aoki (Tohoku University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data