Strain Data Sheet

RBRC09904

Information1

Image
BRC No.RBRC09904
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Has1<tm1Nita>Has2<tm1.1Nita>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Naoki Itano, Kyoto Sangyo Universit. RBRC09746 and RBRC09894 are crossed.
Strain descriptionDouble mutant of Has1 KO and floxed Has2. Single mutant of Has1 KO or floxed Has2 is available as RBRC09746 or RBRC09894, respectively.
Colony maintenance
ReferencesCancer Res., 70(18), 7073-7083(2010). 20823158

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Has2hyaluronan synthase 215Has2<tm1.1Nita>targeted mutation 1.1, Naoki Itano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Has1hyaluronan synthase 117Has1<tm1Nita>targeted mutation 1, Naoki Itano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal17

Information2

Donor DNAMouse Phosphoglycerate kinase (PGK) promoter, E col. Neomycin resistance gene, SV40 PolyA signal, mouse Has1 genomic DNA, Phage P1 loxP sites, yeast FRT recombinase target site, mouse Has2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorNaoki Itano (Kyoto Sangyo University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data