Strain Data Sheet

RBRC09898

Strain Information

Image
BRC No.RBRC09898
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(CAG-Aldh2<*>E504K)02Oht
Former Common nameDAL02 mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shigeo Ohta, Nippon Medical School in 2009. A transgenic construct was injected into fertilized eggs derived from C57BL/6. This line was maintained in C57BL/6N background.
Strain descriptionTransgenic mice overexpressing mutated Aldh2 (E504K) under the control of the CAG promoter. This mutated Aldh2 acts in a dominant-negative manner.
Colony maintenance
References
Efficient selection for high-expression transfectants with a novel eukaryotic vector.
H Niwa, K Yamamura, J Miyazaki
Gene, 108(2):193-9 (1991). 1660837

Metabolic remodeling induced by mitochondrial aldehyde stress stimulates tolerance to oxidative stress in the heart.
Jin Endo, Motoaki Sano, Takaharu Katayama, Takako Hishiki, Ken Shinmura, Shintaro Morizane, Tomohiro Matsuhashi, Yoshinori Katsumata, Yan Zhang, Hideyuki Ito, Yoshiko Nagahata, Satori Marchitti, Kiyomi Nishimaki, Alexander Martin Wolf, Hiroki Nakanishi, Fumiyuki Hattori, Vasilis Vasiliou, Takeshi Adachi, Ikuroh Ohsawa, Ryo Taguchi, Yoshio Hirabayashi, Shigeo Ohta, Makoto Suematsu, Satoshi Ogawa, Keiichi Fukuda
Circ Res., 105, 1118-1127(2009). 19815821

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Aldh2
MGI:99600		aldehyde dehydrogenase 2, mitochondrialUNAldh2 CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
  • alcohol sensitivity, acute(MedGEN)

    • Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal carbohydrate metabolism (MP:0013243)

  • abnormal circulating enzyme level (MP:0001570)

  • decreased body fat mass (MP:0014143)

  • decreased body size (MP:0001265)

  • decreased bone mineral density (MP:0000063)
    • more 6 phenotypes
  • decreased cellular sensitivity to oxidative stress (MP:0008413)

  • decreased myocardial fiber size (MP:0004565)

  • decreased skeletal muscle mass (MP:0004819)

  • increased glutathione peroxidase activity (MP:0011602)

  • kyphosis (MP:0000160)

  • small heart (MP:0002188)
    • Detailed phenotype data

    Ordering Information

    Donor DNAChicken βactin -promoter, Mouse 改変型ALDH2, Rabbit β―Globin　PolyA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Circ Res., 105, 1118-1127(2009).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorShigeo Ohta (Nippon Medical School)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data