Strain Data Sheet

RBRC09896

Information1

Image
BRC No.RBRC09896
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cdh17<tm1Ohn>
Former Common nameCDH17 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Drs. Takeyuki Shimizu, Kazuo Ohnishi and Fritz Melchers. E14.1 ES cells derived from 129P2/OlaHsd were used. C57BL/6 congenic strain by backcrossing to C57BL/6J for 10 generations.
Strain descriptionCdh17 knockout mice. Exons 16-18 were replaced with a neomycin resistance cassette.
Colony maintenance
ReferencesJ. Immunol., 35, 957-963 (2005). 15688343
PLoS One. 10(1): e0117566 (2015). 25612318

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cdh17cadherin 174Cdh17<tm1Ohn>targeted mutation 1, Kazuo Ohnishi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Cdh17 genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Immunol., 35, 957-963 (2005). PLoS One. 10(1):e0117566 (2015).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE
DepositorKazuo Ohnishi (National Institute of Infectious Diseases)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data