Strain Data Sheet

RBRC09893

Information1

Image
BRC No.RBRC09893
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Crh<tm1.1(venus)Ksak>
Former Common namemCrh-Venus-deltaNeo
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Kenji Sakimura at Niigata University, and Keiichi Itoi at Tohoku University. C57BL/6 background.
Strain descriptionVenus was knocked-in at exon 2 of Crh (Corticotropin releasing factor) gene. This strain was generated by crossing RBRC06519 with a Flpe line.
Colony maintenance
ReferencesBrain Struct. Funct., 222, 1705-1732 (2017). 27638512

Health Report

Examination Date / Room / Rack2022/04/25Room:3-CRack:F
2022/02/14Room:3-CRack:F
2021/11/15Room:3-CRack:F
2021/08/16Room:3-CRack:F
2021/05/17Room:3-CRack:F
2021/02/15Room:3-CRack:F
2020/11/16Room:3-CRack:F
2020/08/24Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Crhcorticotropin releasing hormone3Crh<tm1.1(venus)Ksak>targeted mutation 1.1, Kenji Sakimura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)3Venus

Information2

Donor DNAA. coerulescens Venus cDNA, yeast Frt (flippase recombination target) site, mouse Crh genomic DNA
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Brain Struct. Funct., 222, 1705-1732 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
DepositorKeiichi Itoi (Tohoku University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data