Information1 | |
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Image | |
BRC No. | RBRC09893 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | C57BL/6-Crh<tm1.1(venus)Ksak> |
Former Common name | mCrh-Venus-deltaNeo |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | |
Appearance | |
Strain development | Developed by Kenji Sakimura at Niigata University, and Keiichi Itoi at Tohoku University. C57BL/6 background. |
Strain description | Venus was knocked-in at exon 2 of Crh (Corticotropin releasing factor) gene. This strain was generated by crossing RBRC06519 with a Flpe line. |
Colony maintenance | |
References | Brain Struct. Funct., 222, 1705-1732 (2017). 27638512 |
Health Report | |
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Examination Date / Room / Rack | 2022/04/25Room:3-CRack:F 2022/02/14Room:3-CRack:F 2021/11/15Room:3-CRack:F 2021/08/16Room:3-CRack:F 2021/05/17Room:3-CRack:F 2021/02/15Room:3-CRack:F 2020/11/16Room:3-CRack:F 2020/08/24Room:3-CRack:F |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)3Venus |
Information2 | |
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Donor DNA | A. coerulescens Venus cDNA, yeast Frt (flippase recombination target) site, mouse Crh genomic DNA |
Research application | FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Brain Struct. Funct., 222, 1705-1732 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp). |
Depositor | Keiichi Itoi (Tohoku University) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |