Strain Data Sheet


Strain Information

BRC No.RBRC09881
SpeciesMus musculus
Strain nameC57BL/6-Tmem173<em1Tsse>
Former Common nameSTING KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Strain developmentDeveloped by Ken Takashima, Hiroyuki Oshiumi and Tsukasa Seya, Hokkaido University in 2015. C57BL/6 genetic background.
Strain descriptionTmem173 (transmembrane protein 173) mutants generated by the CRISPR/Cas9 technique. 5 bp deletion was introduced at exon 3.Tmem173<em1Tsse>: 5 bp deletion at exon 3.ATGGGACTAGCTGGGCTGGGAGACCCAAGgagatggatgactagtcaggaccttaggccctgaaggacaggagaaccccagaagcatagctgtggatttcttgatgtctacagatgccatactccaacctgcatccagccatcccacggcccagaggtcaccgctccaaatatgtagccctcatctttctggtggccagcctgatgatcctttgggtggcaaaggatccaccaaatcacactctgaagtacctagcacttcacctagcCTCGCacgaacttggactactgttgaaaaacctctgctgtctggctgaagagctgtgccatgtccagtccaggtaaccctctgtggtctccacgatgacttgatacccagcaagtatcacacccttgactcctaaccttggccttccctgaacatcatcagctgagttgggctgggatctgaggctaggctgtcccctgcaggtaccagggcagctactggaaggctgtgcgcgcctgcctgggatgccccatccactgtatggctatgattctactatcgtcttatttctatttcctccaaaacactgctgacatatacctcagttggatgtttGGCCTTCTGGTCCTCTATAAGTCCCTAAGC
Colony maintenance
ReferencesBiochem. Biophy. Res. Comm., 478, 1764-1771 (2016). 27608599

Health Report

Examination Date / Room / Rack2024/04/08Room:3-BRack:J


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tmem173transmembrane protein 17318Tmem173<em1Tsse>endonuclease-mediated mutation 1, Tsukasa Seya

Ordering Information

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9(Addgene plasmid #42230)[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9* (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR)**, E. coli pUC origin] * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan).** E. coli Ampicillin resistance gene was not detected by PCR. Other introduced genes were not tested.
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorTsukasa Seya (Hokkaido University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Maekawa H, Inoue T, Ouchi H, Jao TM, Inoue R, Nishi H, Fujii R, Ishidate F, Tanaka T, Tanaka Y, Hirokawa N, Nangaku M, Inagi R.
Mitochondrial Damage Causes Inflammation via cGAS-STING Signaling in Acute Kidney Injury.
Cell Rep 29(5) 1261-1273.e6(2019) 31665638