Strain Data Sheet

RBRC09881

Information1

Image
BRC No.RBRC09881
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Tmem173<em1Tsse>
Former Common nameSTING KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Ken Takashima, Hiroyuki Oshiumi and Tsukasa Seya, Hokkaido University in 2015. C57BL/6 genetic background.
Strain descriptionTmem173 (transmembrane protein 173) mutants generated by the CRISPR/Cas9 technique. 5 bp deletion was introduced at exon 3.Tmem173<em1Tsse>: 5 bp deletion at exon 3.ATGGGACTAGCTGGGCTGGGAGACCCAAGgagatggatgactagtcaggaccttaggccctgaaggacaggagaaccccagaagcatagctgtggatttcttgatgtctacagatgccatactccaacctgcatccagccatcccacggcccagaggtcaccgctccaaatatgtagccctcatctttctggtggccagcctgatgatcctttgggtggcaaaggatccaccaaatcacactctgaagtacctagcacttcacctagcCTCGCacgaacttggactactgttgaaaaacctctgctgtctggctgaagagctgtgccatgtccagtccaggtaaccctctgtggtctccacgatgacttgatacccagcaagtatcacacccttgactcctaaccttggccttccctgaacatcatcagctgagttgggctgggatctgaggctaggctgtcccctgcaggtaccagggcagctactggaaggctgtgcgcgcctgcctgggatgccccatccactgtatggctatgattctactatcgtcttatttctatttcctccaaaacactgctgacatatacctcagttggatgtttGGCCTTCTGGTCCTCTATAAGTCCCTAAGC
Colony maintenance
ReferencesBiochem. Biophy. Res. Comm., 478, 1764-1771 (2016). 27608599

Health Report

Examination Date / Room / Rack2022/04/11Room:3-BRack:J
2022/01/24Room:3-BRack:J
2021/10/26Room:3-BRack:J
2021/08/16Room:3-DRack:E
2021/07/26Room:3-BRack:J
2021/05/17Room:3-DRack:E
2021/02/15Room:3-DRack:E
2020/11/16Room:3-DRack:E
2020/08/24Room:3-DRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tmem173transmembrane protein 17318Tmem173<em1Tsse>endonuclease-mediated mutation 1, Tsukasa Seya

Information2

Donor DNApX330 plasmid vector
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorTsukasa Seya (Hokkaido University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Maekawa H, Inoue T, Ouchi H, Jao TM, Inoue R, Nishi H, Fujii R, Ishidate F, Tanaka T, Tanaka Y, Hirokawa N, Nangaku M, Inagi R.
Mitochondrial Damage Causes Inflammation via cGAS-STING Signaling in Acute Kidney Injury.
Cell Rep 29(5) 1261-1273.e6(2019) 31665638