Strain Data Sheet

RBRC09880

Strain Information

Image
BRC No.RBRC09880
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Slfn5<em1Tsse>
Former Common nameSLFN5 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yohei Takeda and Tsukasa Seya, Hokkaido University in 2014. C57BL/6 genetic background.
Strain descriptionSlfn5 (schlafen 5) mutants generated by the CRISPR/Cas9 technique. 1bp insertion was introduced at exon 3.Slfn5<em1Tsse>: 1bp insertion at exon 3.AAGCAAACTCAAACAAGCAGTTCCCTGTGGccatcccacagacttagcctctttcgtgaagctggcctcaagctatgtgggataaagggtcaacttcagaaaggagttcttaaaacttcatttcccacctgctccttcttgttctagtctaatggttctatttttctgggtttcaggaaaagatttcagctaaaggcggagaagatgagtttcctggaggatttggaactgaactttgcggaatgtatacaagatggaggaaaagcgacccttggggttaggcaaagggaggaaatggataccacacactgcatgaaacagaacgaaatcatctcgcaagccgtgtgcgctctgctgaattc(T)cggtggcggagtggtcagggttgagattgagaacggagactataattttgagagggatggagttggcttgaatctgccaccattgttcagaaaccatttagacgagatgctgtatggaaagctctttttaatatatgtgagttcgtgggacgtggcagcctctcacgtgaggctcgctaccctgtgctccaatttgtaccacagatgtGGAACATTTACTGAGGTCATGGATCCTGAA
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Slfn5
MGI:1329004		schlafen 511Slfn5<em1Tsse> endonuclease-mediated mutation 1, Tsukasa Seya

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • obsolete no phenotypic analysis (MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNApX330 vector (Streptococcus pyogenes Cas9*, Escherichia coli ampicillin resistance gene, synthetic FLAG tag)* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorTsukasa Seya (Hokkaido University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data