Strain Data Sheet

RBRC09862

Strain Information

Image
BRC No.RBRC09862
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Pgam5<tm1Hijo>
Former Common name
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo. RENKA (C57BL/6) ES cells were used. C57BL/6 genetic background.
Strain descriptionPgam5(phosphoglycerate mutase family member 5) knockout mice.
Colony maintenance
ReferencesEBioMedicine, 5, 82-92 (2016). 27077115
Proc. Natl. Acad. Sci. USA, 106(30):12301-12305 (2009). 19590015

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pgam5phosphoglycerate mutase family member 55Pgam5<tm1Hijo>targeted mutation 1, Hidenori Ichijo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAMouse phosphoglycerate kinase 1 promoter, E.coli neomycin-resistance gene, mouse Pgam5 genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHidenori Ichijo (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data