RBRC09860

Information1

Image
BRC No.RBRC09860
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Thy1-FUS<*>deltaNLS)1Dit
Former Common namedeltaNLF-FUStg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentA human FUS cDNA without a nuclear localization signal was inserted into the pTSC21k vector, which encodes the 8.1 kb murine Thy1.2 expression cassette. This construct was Not I digested to remove vector sequences, co-injected into the pronucleus of fertilized eggs from Slc:BDF1 strain mice. Founders were bred and backcrossed with C57Bl6/J mice to establish transgenic lines.
Strain descriptionA transgenic (tg) mouse overexpressing exogenous FUS without a nuclear localization signal (ΔNLS-FUS) develop progressive spastic motor deficits and neuronal loss in the motor cortex. The ΔNLS-FUS protein was restricted to the cytoplasm and formed ubiquitin/p62-positive aggregates. Endogenous FUS expression, nuclear localization, and splicing activity were not altered, indicating that mislocated FUS is sufficient for proteinopathy. RNA-sequence analysis revealed specific transcriptome alterations, including genes regulating dynein-associated molecules and endoplasmic reticulum stress.
Colony maintenanceFounders were bred and backcrossed with C57Bl6/J mice to establish transgenic lines.
ReferencesBrain, 139, 2380-2394 (2016). 27368346

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
null

Information2

Donor DNAマウス Thy-1 promoterとマウスThy-1のexon3,4, ヒト human FUS cDNA without a nuclear localization signalを挿入, マウス Thy-1exon3,4
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Brain, 139, 2380-2394 (2016).
DepositorDaisuke Ito (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data