Strain Data Sheet

RBRC09859

Information1

Image
BRC No.RBRC09859
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CMV-cat,-SDHA<*>Y215F)1Hom
Former Common nameCATFL-SDHAY215F Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yoshimi Homma, Fukushima Medical University in 2012. C57BL/6 genetic background.
Strain descriptionTransgenic mice expressing mutated SDHA (Succinate dehydrogenase A, Y215F) in a Cre-dependent manner. Floxed cat (chloramphenicol acetyltransferase) gene and SDHA(Y215F) are driven by the CMV promoter.
Colony maintenance
ReferencesEur. J. Immunol., 47, 406-418 (2017). 27883180
Biochem. J., 447(2), 281-289 (2012). 22823520

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)UNFLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SDHAsuccinate dehydrogenase complex, subunit A, flavoprotein (Fp) (human)UNSDHA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
catE. coli chloramphenicol acetyltransferase (CAT)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxPCMV promoter, rabbit beta-globin intron, rabbit beta-globin poly A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly Apolyadenylic acid additional signalUNpoly A

Information2

Donor DNACytomegalovirus CMV promoter, rabbit beta globin intron, Phage P1 loxP sites, E. coli chloramphenicol acetyltransferase (CAT) gene, rabbit beta globin polyA, Human SDHA gene cDNA, FLAG epitope tag(synthetic), pA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Eur. J. immunol., 47, 406-418 (2017).
DepositorYoshimi Honma (Fukushima Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data