Strain Data Sheet


Strain Information

BRC No.RBRC09809
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Sharpin<tm1.1Kiwa>
Former Common nameSHARPIN conditional KO
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Strain developmentDeveloped by Kazuhiro Iwai, Graduate School of Medicine and Faculty of Medicine Kyoto University. Bruce4 ES cell line (B6.Cg-Thy1) was used. FRT-flanked Neo gene was removed by crossing a FLP strain. C57BL/6 congenic line.
Strain descriptionFloxed Sharpin(SHANK-associated RH domain interacting protein)mice. Exon 3 to 9 encoding UBL and NZF domains are flanked by loxP sites.
Colony maintenance
ReferencesNat. Commun., 10(1) 3878 (2019). 31462647

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SharpinSHANK-associated RH domain interacting protein15Sharpin<tm1.1Kiwa>targeted mutation 1.1, Kazuhiro Iwai

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Ordering Information

Donor DNAYeast FRT (flippase recombination target) site, Phage P1 loxP sites, mouse Sharpin genomic DNA
Research applicationCre/loxP system
Dermatology Research
FLP/frt system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 10(1) 3878 (2019).The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKazuhiro Iwai (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data