Strain Information | |
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Image | |
BRC No. | RBRC09808 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | C57BL/6-Rnf31<tm2.1Kiwa> |
Former Common name | HOIP conditional KO |
H-2 Haplotype | |
ES Cell line | JM8 [C57BL/6N] |
Background strain | |
Appearance | |
Strain development | Developed by Kazuhiro Iwai, Graduate School of Medicine and Faculty of Medicine Kyoto University. JM8 ES cell line (C57BL/6N) was used. FRT-flanked Neo gene was removed by crossing a FLP strain. C57BL/6 genetic background. |
Strain description | Floxed Rnf31(ring finger protein 31,HOIP)mice. Exon 7 to 11 encoding ZF and UBA domains are flanked by loxP sites. |
Colony maintenance | |
References | Modulation of autoimmune pathogenesis by T cell-triggered inflammatory cell death. Sasaki K, Himeno A, Nakagawa T, Sasaki Y, Kiyonari H, Iwai K Nat. Commun., 10(1) 3878 (2019). 31462647 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | 14 | Frt | ||||
Rnf31 MGI:1934704 | ring finger protein 31 | 14 | Rnf31<tm2.1Kiwa> | targeted mutation 2.1, Kazuhiro Iwai | |||
loxP | phage P1 loxP | 14 | loxP | ||||
loxP | phage P1 loxP | 14 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Yeast FRT (flippase recombination target) site, Phage P1 loxP sites, mouse Rnf31 genomic DNA |
Research application | Cre/loxP system FLP/frt system Immunology and Inflammation Research |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 10(1) 3878 (2019). The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Kazuhiro Iwai (Kyoto University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Jimbo K, Hattori A, Koide S, Ito T, Sasaki K, Iwai K, Nannya Y, Iwama A, Tojo A, Konuma T. Genetic deletion and pharmacologic inhibition of E3 ubiquitin ligase HOIP impairs the propagation of myeloid leukemia. Leukemia (2022) 36352193 |