Strain Data Sheet

RBRC09806

Strain Information

Image
BRC No.RBRC09806
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-cat,-EGFP)39Miya
Former Common nameCAG-CAT-EGFP mice, CAG-LSL-EGFP mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Jun-ich Miyazaki in 1999. Transgene was injected into BDF1, and then mice were backcrossed to C57BL/6 for more than 30 generations.
Strain descriptionTransgenic reporter mice for Cre recombinase. Floxed cat gene can be excised by Cre recombinase, resulting in EGFP expression.
Colony maintenance
ReferencesFEBS Lett., 470, 263-268 (2000). 10745079

Health Report

Examination Date / Room / Rack2024/03/11Room:3-5Rack:D
2023/12/11Room:3-5Rack:D
2023/09/11Room:3-5Rack:D
2023/06/13Room:3-5Rack:D
2023/03/13Room:3-5Rack:D
2022/12/12Room:3-5Rack:D
2022/09/12Room:3-5Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
catE. coli chloramphenicol acetyltransferase (CAT)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP sites, E. coli Chloramphenicol acetyltransferase gene, jellyfish Enhanced green fluorescent protein cDNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Letters, 470, 263-268 (2000).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for- profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorJun-ichi Miyazaki (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Baba Y, Onishi-Sakamoto S, Ide K, Nishifuji K.
Nestin is a marker of unipotent embryonic and adult progenitors differentiating into an epithelial cell lineage of the hair follicles.
Sci Rep 12(1) 17820(2022) 36280775