Strain Data Sheet

RBRC09802

Information1

Image
BRC No.RBRC09802
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129(FVB)-Sptlc2<tm1.1Yhir>
Former Common name
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yoshio Hirabayashi, RIKEN Brain Science Institute in collaboration with Osaka University. R1 ES cell line [(129X1/SvJ x 129S1/Sv)F1] was used. Exon 3 was replaced with a fragment loxP-neo-loxP-Exon3-loxP. Neo fragment was removed by crossing with an EIIa-Cre mouse strain.
Strain descriptionSptlc2(serine palmitoyltransferase, long chain base subunit 2)floxed mice. Homozygous null mutants are embryonically lethal.
Colony maintenance
ReferencesExp. Anim., 58, 515-524 (2009). 19897935
Cell Metab., 24(6):820-834 (2016). 27818258

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sptlc2serine palmitoyltransferase, long chain base subunit 212Sptlc2<tm1Yhir>targeted mutation 1, Yoshio Hirabayashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP12loxP

Information2

Donor DNAPhage P1 loxP sites, mouse Sptlc2 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 58, 515-524 (2009).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorYoshio Hirabayashi (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Jun 2017
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data