Strain Data Sheet

RBRC09797

Information1

Image
BRC No.RBRC09797
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ano6<tm1.2Naga>
Former Common nameTMEM16F KO
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shigekazu Ngata, Jun Suzuki and Toshihiro Fujii at Osaka University in 2013. Bruce 4 ES cell line was used. Exon 2 and Neomycin resistance gene were removed by crossing CAG-Cre mice.
Strain descriptionAno6(anoctamin 6, Tmem16f) knockout mice.
Colony maintenance
ReferencesNature, 468, 834-838 (2010). 21107324
J. Biol. Chem., 288, 13305-13316 (2013). 23532839

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ano6anoctamin 615Ano6<tm1.2Naga>targeted mutation 1.2, Shigekazu Nagata

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Information2

Donor DNAPhage P1 loxP site, mouse Ano6 genome DNA
Research applicationCre/loxP system
Mouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 288, 13305-13316 (2013).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorShigekazu Nagata (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data