Strain Data Sheet

RBRC09796

Information1

Image
BRC No.RBRC09796
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ano6<tm1.1Naga>
Former Common nameTMEM16F Flox
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Shigekazu Ngata, Jun Suzuki and Toshihiro Fujii at Osaka University in 2013. Bruce 4 ES cell line was used. Neomycin resistance gene was removed by crossing CAG-FLPe mice. A pair of loxP are flanking exon 2.
Strain descriptionFloxed mice for Ano6(anoctamin 6, Tmem16f).
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 112, 12800-12805 (2015). 26417084
Nature, 468, 834-838 (2010). 21107324
J. Biol. Chem., 288, 13305-13316 (2013). 23532839

Health Report

Examination Date / Room / Rack2022/05/23Room:4-ARack:E
2022/03/07Room:4-ARack:E
2021/12/06Room:4-ARack:E
2021/10/01Room:4-ARack:E
2021/09/08Room:4-ARack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ano6anoctamin 615Ano6<tm1.1Naga>targeted mutation 1.1, Shigekazu Nagata

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Information2

Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Ano6 genome DNA
Research applicationCre/loxP system
FLP/frt system
Mouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 112, 12800-12805 (2015). J. Biol. Chem., 288, 13305-13316 (2013).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorShigekazu Nagata (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data