RBRC09792

Information1

Image
BRC No.RBRC09792
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Cabyr<em1Osb>
Former Common nameCabyr<+17/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesJ. Cell Sci. 2016 Dec 1;129(23):4379-4387. Epub 2016 Oct 19. 27802166

Health Report

Examination Date / Room / RackRoom:3-CRack:B
2020/08/24Room:3-CRack:B
2020/06/23Room:3-CRack:B
2020/06/15Room:3-CRack:B
2020/06/01Room:3-CRack:B
2020/02/17Room:3-CRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cabyrcalcium-binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2)18Cabyr<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Information2

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9* (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Cabyr gene[* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.]
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci. 2016 Dec 1;129(23):4379-4387. Epub 2016 Oct 19. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Category I MTA: CRISPR/Cas9 genome edited bioresources ( Japanese / English )
Lab HP

BRC mice in Publications

No Data