Strain Data Sheet
RBRC09792
Strain Information | |
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| Image | |
| BRC No. | RBRC09792 |
| Type | CRISPR/Cas9 |
| Species | Mus musculus |
| Strain name | B6D2-Cabyr<em1Osb> |
| Former Common name | Cabyr<+17/wt> |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2014. BDF1 background. |
| Strain description | Cabyr mutant mice generated by the CRISPR/Cas9 technique. 17 bp insertion in Exon 2Cabyr<em1Osb> (Inserted sequence):tgtgcattagtaagcagtgggttttattttatatgttactagaataaatagagattagaagtaaagctaataatggtaatagatttggttttagaaaccccaattaaataagaatgctcagtaaatctaatgtacagtataagactttgtttctcttcattacagTTGAAATTACAGACATCTTGCCAAAATGATTTCTTCAAAGCCCAGACTTGTTGTACCCTATGGCCTCAAGACTCTGC(agagtaaagctttagtt)TTGAGGGAGTTAGCAGAGCCATTCTCAAAACCAATCCAACAAACATCACCCAGTTTGCAGCCGTTTATTTTAAAGAACTCATTGTGTTTAGAGAAGgtttgtactgctttagatctgtatttcctgctttacagtcatcttaaatggaaatccatctctatttgacttcctatgcttattcctccagggaattcgtctctagatataaaagatctgattaaacagtttcaccagatgaaaggtaagtgcgacggagacgcccacttcagcagggtcgtca |
| Colony maintenance | |
| References | CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa. Young S A, Miyata H, Satouh Y, Aitken R J, Baker M A, Ikawa M J. Cell Sci. 2016 Dec 1;129(23):4379-4387. Epub 2016 Oct 19. 27802166 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Cabyr | calcium binding tyrosine phosphorylation regulated | 18 | Cabyr | endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University | |||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | more 5 phenotypes |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9* (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli pUC origin], Mouse a part of Cabyr gene[* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). E. coli Ampicillin resistance gene was not detected by PCR. Other introduced genes were not tested. |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci. 2016 Dec 1;129(23):4379-4387. Epub 2016 Oct 19. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |