RBRC09776

Information1

Image
BRC No.RBRC09776
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Efcab3<tm1a(KOMP)Osb>/40
Former Common nameEfcab3#40
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 113(28):7704-10 (2016). 27357688

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Efcab3EF-hand calcium binding domain 311Efcab3<tm1a(KOMP)Osb>targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)11En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly Apolyadenylic acid additional signal11poly A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly Apolyadenylic acid additional signal11poly A

Information2

Donor DNA[chicken beta-actin promoter, rabbit beta-globin polyadenylation signal,CMV CMV IE enhancer, mouse proacrosin signal peptide, mouse acrosin N-ternimal peptide, jellyfish GFP cDNA, mouse acrosin promoter], Yeast FRT sites, mouse En2 splice acceptor (En2 intron2/exon 3 splice acceptor), EMCV Internal ribosomal entry site (IRES), E. coli. lacZ, SV40 polyadenylation site, Phage P1 loxP sites, human Beta-actin promoter, E. coli. Neomycin phosphotransferase gene (neoR) , mouse Efcab3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proce Natl Acad Sci USA. 2016, 113 (28): 7704-10RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP

BRC mice in Publications

No Data