RBRC09768

Information1

Image
BRC No.RBRC09768
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm2(CAG-HIST1H2BJ)Shna>/24
Former Common nameflagH2B #24, #19
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesSci Rep., 8(1):1143 (2018). 29348483

Health Report

Examination Date / Room / Rack2019/10/21Room:3-CRack:F
2019/08/26Room:3-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2(CAG-HIST1H2BJ)Shna>targeted mutation 2, Shinichi Nakagawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
FLAGFLAG tag (synthetic)6FLAG

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HIST1H2BJhistone cluster 1, H2bj (human)6HIST1H2BJCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA6

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Enterobacteria phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 polyA, FLAG tag sequence (synthetic), human histone cluster 1, H2bj (HIST1H2BJ), bovine growth hormone polyA signal, mouse ROSA26 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Type Specific Survey of Epigenetic Modifications by Tandem Chromatin Immunoprecipitation Sequencing. Preprint at http://www.biorxiv.org/content/early/2017/07/14/163568In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorShinichi Nakagawa (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data