RBRC09758

Information1

Image
BRC No.RBRC09758
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Chd8<tm1Kei>
Former Common nameChd8/Duplin KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeposited by Keiichi Nakayama, Medical Institute of Bioregulation, Kyusyu University in 2016. E14(129P2/OlaHsd) ES cell line was used. Mixed genetic background in C57BL/6 and 129P2.
Strain descriptionChd8 (chromodomain helicase DNA binding protein 8) knockout mice. Homozygous mutants are lethal. Heterozygous mutant displays autistic like behavior. All exons were replaced by loxP-neo cassette. Mouse strain carrying deletion of only long isoform of Chd8 (Chd8l) is available as RBRC09759.
Colony maintenance
ReferencesMol. Cell. Biol., 24: 8386-8394 (2004). 15367660
Nature, 537(7622):675-679 (2016). 27602517
Nature Cell Biol., 11: 172-182 (2009). 19151705

Health Report

Examination Date / Room / Rack2018/04/16Room:3-BRack:I
2018/02/19Room:3-BRack:I
2017/12/18Room:3-BRack:I
2017/10/16Room:3-BRack:I
2017/08/21Room:3-BRack:I
2017/06/19Room:3-BRack:I
2017/04/17Room:3-BRack:I
2017/02/20Room:3-BRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Chd8chromodomain helicase DNA binding protein 814Chd8<tm1Kei>targeted mutation 1, Keiichi I Nakayama

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 PolyA signal14

Information2

Donor DNABacteriophage P1 loxP sites, Mouse phosphoglycerate kinase promoter, E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Chd8 genomic DNA
Research applicationCre/loxP system
Mouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKeiichi Nakayama (Kyushu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Nov 2018

BRC mice in Publications

No Data