Strain Data Sheet

RBRC09742

Strain Information

Image
BRC No.RBRC09742
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Ambp<tm1Kkm>
Former Common nameBikunin knockout mouse, UTI knockout mice, ITILC knockout mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Lisheng Zhuo and Koji Kimata, Aichi Medical University in 2000. E14 ES cell was used. C57BL/6N congenic line. Other congenic lines in C3H/HeSlc(RBRC09743), DBA1/JJms(RBRC09744)or BALB/cCrSlc(RBRC09745)are also available.
Strain descriptionAmbp(alpha 1 microglobulin/bikunin)knockout mice. A neo cassette replaced exons coding the Bikunin protein, but unchanged exons coding the alpha 1 microglobulin protein. Homozygous females are infertile.
Colony maintenance
References
Kupffer cells and activation of endothelial TLR4 coordinate neutrophil adhesion within liver sinusoids during endotoxemia.
McDonald B, Jenne C N, Zhuo L, Kimata K, Kubes P
Am. J. Physiol. Gastrointest. Liver. Physiol., 305, G797-806 (2013). 24113769

Defect in SHAP-hyaluronan complex causes severe female infertility. A study by inactivation of the bikunin gene in mice.
Zhuo L, Yoneda M, Zhao M, Yingsung W, Yoshida N, Kitagawa Y, Kawamura K, Suzuki T, Kimata K
J. Biol. Chem., 276, 7693-7696 (2001). 11145954

Deficiency in the serum-derived hyaluronan-associated protein-hyaluronan complex enhances airway hyperresponsiveness in a murine model of asthma.
Zhu L, Zhuo L, Kimata K, Yamaguchi E, Watanabe H, Aronica M A, Hascall V C, Baba K
Int. Arch. Allergy Imm., 153, 223-233 (2010). 20484920

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 Late and early polyA signal4
Ambpalpha 1 microglobulin/bikunin precursor4Ambptargeted mutation 1, Koji Kimata
neoneomycin resistance gene (E. coli)4herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal maternal nurturing(MP:0001386)

  • abnormal oocyte morphology(MP:0001125)

  • absent cumulus expansion(MP:0009374)

  • decreased litter size(MP:0001935)

  • failure of zygotic cell division(MP:0003406)
    • more 3 phenotypes
  • female infertility(MP:0001926)

  • impaired fertilization(MP:0000242)

  • reduced female fertility(MP:0001923)
    • Detailed phenotype data

    Ordering Information

    Donor DNAHSV thymidine kinase promoter, E coli Neomycin resistance gene, HSV thymidine kinase polyA signal, SV40 Late and early polyA signal (a 139bp fragment between HpaI and BamHI sites in pMAMneo-S vector(Clontech), mouse Ambp genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorKoji Kimata (Aichi Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data