Strain Data Sheet

RBRC09740

Information1

Image
BRC No.RBRC09740
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ddhd1<tm1b(KOMP)Katn>
Former Common name
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Katsuko Tani, Tokyo University of Pharmacy and Life Sciences. TT2 (B6/CBA) ES cell line was used. C57BL/6J congenic line.
Strain descriptionDdhd1 (DDHD domain containing 1) knockout mice. RBRC09738 is a strain in which FRT-flanked lacZ/neomycyin fusion gene (geo) followed by a loxP site was inserted in front of exon 8 and another loxP site was inserted after the exon 8. RBRC09740 is a strain in which RBRC09738 was futher crossed with CAG-Cre to delete the exon 8, and then backcrossed to C57BL/6J. Homozygous male mice showed subfertility. A strain in mixed genetic background of C57BL/6 and CBA is available as RBRC09739.
Colony maintenance
ReferencesJ. Biol. Chem., 289, 11497-11511 (2014). 24599962

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ddhd1DDHD domain containing 114Ddhd1<tm1b(KOMP)Katn>targeted mutation 1b, Katsuko Tani

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)14

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA14

Information2

Donor DNAyeast FRT (flipase recombination target) sites, Mouse En2 intron 2/exon 3 splice acceptor sequence, E.coli lacZ, E.coli neo, SV40 PolyA, Bacteriophage P1 loxP site, mouse Ddhd1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 289, 11497-11511 (2014).RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Within a period of TWO years after the first paper concering of the BIOLOGICAL RESOURCE is published, the RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
DepositorMitsuo Tagaya (Tokyo University of Pharmacy and Life Sciences)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data