Strain Data Sheet

RBRC09739

Strain Information

Image
BRC No.RBRC09739
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Ddhd1<tm1b(KOMP)Katn>
Former Common namePA-PLA1 KO mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Katsuko Tani, Tokyo University of Pharmacy and Life Sciences. TT2 (B6/CBA) ES cell line was used. Mixed genetic background of C57BL/6 and CBA.
Strain descriptionDdhd1 (DDHD domain containing 1) knockout mice. RBRC09738 is a strain in which FRT-flanked lacZ/neomycyin fusion gene (geo) followed by a loxP site was inserted in front of exon 8 and another loxP site was inserted after the exon 8. RBRC09739 is a strain in which RBRC09738 was futher crossed with CAG-Cre to delete the exon 8. Homozygous male mice showed subfertility. C57BL/6J congenic line is available as RBRC09740.
Colony maintenance
References
Phosphatidic acid (PA)-preferring phospholipase A1 regulates mitochondrial dynamics.
Baba T, Kashiwagi Y, Arimitsu N, Kogure T, Edo A, Maruyama T, Nakao K, Nakanishi H, Kinoshita M, Frohman M A, Yamamoto A, Tani K
J. Biol. Chem., 289, 11497-11511 (2014). 24599962

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA14
  • hereditary spastic paraplegia 28(MedGEN)
    • Ddhd1DDHD domain containing 114Ddhd1targeted mutation 1b, Katsuko Tani
  • hereditary spastic paraplegia 28(MedGEN)
    • En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)14En2
  • hereditary spastic paraplegia 28(MedGEN)
    • Frtyeast FRT (flippase recombination target) site14Frt
  • hereditary spastic paraplegia 28(MedGEN)
    • Frtyeast FRT (flippase recombination target) site14Frt
  • hereditary spastic paraplegia 28(MedGEN)
    • lacZbeta-galactosidase (E. coli)14
  • hereditary spastic paraplegia 28(MedGEN)
    • loxPphage P1 loxP14loxP
  • hereditary spastic paraplegia 28(MedGEN)
    • neoneomycin resistance gene (E. coli)14
  • hereditary spastic paraplegia 28(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal sperm motility(MP:0002674)

  • decreased testis weight(MP:0004852)

  • oligozoospermia(MP:0002687)

  • reduced male fertility(MP:0001922)

  • teratozoospermia(MP:0005578)
    • Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flipase recombination target) sites, Mouse En2 intron 2/exon 3 splice acceptor sequence, E.coli lacZ, E.coli neo, SV40 PolyA, Bacteriophage P1 loxP site, mouse Ddhd1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 289, 11497-11511 (2014).RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Within a period of TWO years after the first paper concering of the BIOLOGICAL RESOURCE is published, the RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
    DepositorMitsuo Tagaya (Tokyo University of Pharmacy and Life Sciences)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data