Strain Data Sheet

RBRC09737

Strain Information

Image
BRC No.RBRC09737
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Sec23ip<tm1.1Katn>
Former Common namep125 KO mice B6 backcross
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Katsuko Tani, Tokyo University of Pharmacy and Life Sciences. TT2 (B6/CBA) ES cell line was used. C57BL/6J-congenic line.
Strain descriptionSec23ip (別名p125) knockout mice. Exon 1 of the gene was replaced with loxp-flanked neo gene. The floxed neo gene was removed by crossing to CAG-Cre line. Male homozygotes tend to be infertile.
Colony maintenance
References
p125/Sec23-interacting protein (Sec23ip) is required for spermiogenesis.
Arimitsu N, Kogure T, Baba T, Nakao K, Hamamoto H, Sekimizu K, Yamamoto A, Nakanishi H, Taguchi R, Tagaya M, Tani K
FEBS Lett., 585, 2171-2176 (2011). 21640725

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sec23ipSec23 interacting protein7Sec23iptargeted mutation 1.1, Katsuko Tani
loxPphage P1 loxP7loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal spermatid morphology(MP:0006380)

  • absent acrosome(MP:0008839)

  • globozoospermia(MP:0002686)

  • impaired fertilization(MP:0000242)

  • reduced male fertility(MP:0001922)
    • more 1 phenotypes
  • teratozoospermia(MP:0005578)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 Phage loxP site, mouse Sec23ip genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Letters, 585, 2171-2176 (2011).RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Within a period of TWO years after the first paper concering of the BIOLOGICAL RESOURCE is published, the RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorMitsuo Tagaya (Tokyo University of Pharmacy and Life Sciences)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data