Strain Data Sheet

RBRC09735

Information1

Image
BRC No.RBRC09735
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Tg(CAG-EGFP,-IL6/mCherry)56Kenw
Former Common nameLGL-IL6 (Li56)
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Ken Watanabe, National Center for Geriatrics and Gerontology in 2015. C57BL/6N genetic background.
Strain descriptionTransgenic mouse line to express Human IL-6 in a Cre-dependent manner. CAG promoter drives loxP-GFP-pA-loxP-IL-6-2A-mCherry-pA. Another transgenic subline of RBRC09735 is available as RBRC09734.
Colony maintenance
ReferencesExp. Anim., 65(4):455-463 (2016). 27349442

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IL6interleukin 6 (human)UNIL6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)UNT2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)UNmCherry

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyAUN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mouse Proacrosin signal sequenceUN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Thy-1 GPI anchor signal sequenceUN

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage loxP sites, Aequorea victoria EGFP cDNA, SV40 PolyA signal, Human IL-6 cDNA, Mouse Proacrosin signal sequence; Thy-1 GPI anchor signal sequence, Thosea asigna virus 2A peptide (T2A), Discosoma sp. mCherry cDNA, Bovine GH polyA signal
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
covid19_onset
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mori et al. Exp. Anim. (2016)
DepositorKen Watanabe (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
  6. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data